Presentation Type
Poster
Location
Walker Conference Center
Room Number
Room A
Start Date
26-3-2024 7:10 PM
End Date
26-3-2024 7:30 PM
Abstract
Recent studies show that up 27-36% of all infections are polymicrobial, involving more than one bacteria species. In all categories, polymicrobial infections are often more virulent and harmful to the patient. Since different types of bacteria can communicate with each other through quorum-sensing when in the presence of each other, they can display different genomic characteristics. Further research into polymicrobial infections needs to be done because these changing characteristics can prevent treatment from working, such as antibiotics or bacteriophage therapy. This experiment focused on the co-culture between the two bacteria, Pseudomonas aeruginosa and Staphylococcus aureus, which can be found co-existing in Cystic fibrosis patients. With an HRQT assay, the two experiments were conducted: comparing phage’s capability when a bacterium was put in another bacteria’s filtrate and a phage cocktail’s ability when put into a co-culture of Pseudomonas and Staphylococcus. The results in the first experiment showed a significant influence of a bacteria’s filtrate on both the growth of other bacteria and the ability of phage to lyse. In the second experiment, the phage cocktail was still able to kill the bacteria for 6 more hours than the control. When comparing the phage capability between the two experiments, the results emphasize the importance of quorum-sensing between bacteria.
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Vitro Bacteriophage Treatment for a Staphylococcus aureus and Pseudomonas aeruginosa Co-culture
Walker Conference Center
Recent studies show that up 27-36% of all infections are polymicrobial, involving more than one bacteria species. In all categories, polymicrobial infections are often more virulent and harmful to the patient. Since different types of bacteria can communicate with each other through quorum-sensing when in the presence of each other, they can display different genomic characteristics. Further research into polymicrobial infections needs to be done because these changing characteristics can prevent treatment from working, such as antibiotics or bacteriophage therapy. This experiment focused on the co-culture between the two bacteria, Pseudomonas aeruginosa and Staphylococcus aureus, which can be found co-existing in Cystic fibrosis patients. With an HRQT assay, the two experiments were conducted: comparing phage’s capability when a bacterium was put in another bacteria’s filtrate and a phage cocktail’s ability when put into a co-culture of Pseudomonas and Staphylococcus. The results in the first experiment showed a significant influence of a bacteria’s filtrate on both the growth of other bacteria and the ability of phage to lyse. In the second experiment, the phage cocktail was still able to kill the bacteria for 6 more hours than the control. When comparing the phage capability between the two experiments, the results emphasize the importance of quorum-sensing between bacteria.