Presentation Type
Poster
Location
Walker Conference Center and Virtual
Room Number
Room A
Start Date
8-4-2025 7:10 PM
End Date
8-4-2025 7:30 PM
Abstract
Photodynamic Therapy (PDT) is a novel method of cancer treatment. It uses light, oxygen, and a photosensitizer to target and kill malignant cancer cells. In this research, a novel porphyrin, ZnTPP-6AH, has been synthesized for use as a possible photosensitizer for PDT treatment of A549 lung cancer cells. This novel compound is synthesized by the coupling of ZnTPPC with 6-amino-1-hexanol. The new water-soluble zinc porphyrin was purified using Sephadex LH-20 and G-50 column chromatography. After purification, the porphyrin was characterized using ultraviolet-visible (UV-vis), nuclear magnetic resonance (NMR), and infrared (IR) spectroscopies. The final purity of this compound was then determined using HPLC. Additionally, cyclovoltammetry (CV) and fluorescence spectroscopy were carried out on the ZnTPP-6AH. Finally, cell viability using A549 lung cancer cells with ZnTPP-6AH as the photosensitizer was determined using both PrestoBlue and MTT assays.
Keywords
Madison Goff, Photodynamic Therapy, Porphyrin, Lung Cancer
Arkansas Women In STEM Conference
Walker Conference Center and Virtual
Photodynamic Therapy (PDT) is a novel method of cancer treatment. It uses light, oxygen, and a photosensitizer to target and kill malignant cancer cells. In this research, a novel porphyrin, ZnTPP-6AH, has been synthesized for use as a possible photosensitizer for PDT treatment of A549 lung cancer cells. This novel compound is synthesized by the coupling of ZnTPPC with 6-amino-1-hexanol. The new water-soluble zinc porphyrin was purified using Sephadex LH-20 and G-50 column chromatography. After purification, the porphyrin was characterized using ultraviolet-visible (UV-vis), nuclear magnetic resonance (NMR), and infrared (IR) spectroscopies. The final purity of this compound was then determined using HPLC. Additionally, cyclovoltammetry (CV) and fluorescence spectroscopy were carried out on the ZnTPP-6AH. Finally, cell viability using A549 lung cancer cells with ZnTPP-6AH as the photosensitizer was determined using both PrestoBlue and MTT assays.
