The Development of a New Water-Soluble Zinc Porphyrin, ZnTPP-2MP, as a Photodynamic Therapy Agent
Presentation Type
Thesis
Department
Biology
Location
Walker Conference A
Description
Photodynamic therapy is a new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. This research tested the use of ZnTPP-2MP as a photosensitizer using white light as a source of possible treatment of the A549 cancer cell line, with preliminary testing on MYC-22 and other G-quadruplex DNA samples. ZnTPP-2MP was synthesized by the reaction of the porphyrin, ZnTPPC, with 2-amino-2-methyl-1-propanol. Following synthesis, the new porphyrin was purified by column chromatography using Sephadex LH-20 and G-50. To confirm the identity and structure of the product, ZnTPP-2MP was analyzed through nuclear magnetic resonance (NMR), infrared (IR), ultraviolet-visible (UV-vis) and fluorescence spectroscopies. Purity of the final compound was determined using HPLC. A reactive oxygen species (ROS) assay was performed using the novel porphyrin, as well as an MTT assay for cell viability.
Creative Commons License
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The Development of a New Water-Soluble Zinc Porphyrin, ZnTPP-2MP, as a Photodynamic Therapy Agent
Walker Conference A
Photodynamic therapy is a new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. This research tested the use of ZnTPP-2MP as a photosensitizer using white light as a source of possible treatment of the A549 cancer cell line, with preliminary testing on MYC-22 and other G-quadruplex DNA samples. ZnTPP-2MP was synthesized by the reaction of the porphyrin, ZnTPPC, with 2-amino-2-methyl-1-propanol. Following synthesis, the new porphyrin was purified by column chromatography using Sephadex LH-20 and G-50. To confirm the identity and structure of the product, ZnTPP-2MP was analyzed through nuclear magnetic resonance (NMR), infrared (IR), ultraviolet-visible (UV-vis) and fluorescence spectroscopies. Purity of the final compound was determined using HPLC. A reactive oxygen species (ROS) assay was performed using the novel porphyrin, as well as an MTT assay for cell viability.
Comments
The research for this presentation was done in collaboration with Dr. Joe Bradshaw. This presentation is currently embargoed.