Developing a Protocol for Bacteriophage Guided Evolution
Presentation Type
Thesis
Department
Biology
Location
Walker Conference Center C
Description
Bacteriophage therapy is being explored as an alternative to antibiotic use due to an increase in antibiotic resistance. Expansion of phage host ranges is important for future phage therapy. A protocol was developed based upon the Appelmans Protocol to expand the host range of selected Gordonia bacteriophage. A spectrophotometer was used to determine lysis in a 96-well plate and confirm the success of the methods. A 100 phage to 1 bacteria concentration ratio was found to be effective. Two methods of bacteria concentration calculations were compared, one using a countable plate and the other using a spectrophotometer. The findings of the experiments were applied to a final protocol, which is followed by instructions on publishing research.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Developing a Protocol for Bacteriophage Guided Evolution
Walker Conference Center C
Bacteriophage therapy is being explored as an alternative to antibiotic use due to an increase in antibiotic resistance. Expansion of phage host ranges is important for future phage therapy. A protocol was developed based upon the Appelmans Protocol to expand the host range of selected Gordonia bacteriophage. A spectrophotometer was used to determine lysis in a 96-well plate and confirm the success of the methods. A 100 phage to 1 bacteria concentration ratio was found to be effective. Two methods of bacteria concentration calculations were compared, one using a countable plate and the other using a spectrophotometer. The findings of the experiments were applied to a final protocol, which is followed by instructions on publishing research.
Comments
This presentation is currently embargoed. It will be available May 2029.