Characterization of Bacteriophage Infection of Gordonia species Utilizing an Optical Density-based Approach
Presentation Type
Thesis
Department
Biology
Location
Walker Conference Center C
Description
Bacteriophages are viruses that infect and replicate within bacterial hosts; phage-host interactions, including successful infections, play a role in shaping bacterial populations. This research aims to describe bacteriophage infection in Gordonia, a soil actinomycete. The infection efficacy of a phage is determined by analyzing the growth of infected bacteria and comparing it to an uninfected host. Optical density measurements are used to describe bacterial growth, with virus infection metrics determined by analyzing optical density values of uninfected and infected cultures over time. These experiments evaluated temperate Gordonia phages Goib, GrandSlam, and Ruthy on host species Gordonia terrae CAG3, Gordonia lacunae, and Gordonia rubripertincta. Preliminary results confirm findings previously observed in phage lambda, that temperate phages “choose” lysogeny over lysis at a high multiplicity of infection. This research will provide insights into how phage infection dynamics vary among different Gordonia host species, contributing to the understanding of phage-host interactions. The outcomes have implications for fields such as microbial ecology and phage biology, furthering our knowledge of phage infection dynamics and bacteriophage-host interactions.
Creative Commons License
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Characterization of Bacteriophage Infection of Gordonia species Utilizing an Optical Density-based Approach
Walker Conference Center C
Bacteriophages are viruses that infect and replicate within bacterial hosts; phage-host interactions, including successful infections, play a role in shaping bacterial populations. This research aims to describe bacteriophage infection in Gordonia, a soil actinomycete. The infection efficacy of a phage is determined by analyzing the growth of infected bacteria and comparing it to an uninfected host. Optical density measurements are used to describe bacterial growth, with virus infection metrics determined by analyzing optical density values of uninfected and infected cultures over time. These experiments evaluated temperate Gordonia phages Goib, GrandSlam, and Ruthy on host species Gordonia terrae CAG3, Gordonia lacunae, and Gordonia rubripertincta. Preliminary results confirm findings previously observed in phage lambda, that temperate phages “choose” lysogeny over lysis at a high multiplicity of infection. This research will provide insights into how phage infection dynamics vary among different Gordonia host species, contributing to the understanding of phage-host interactions. The outcomes have implications for fields such as microbial ecology and phage biology, furthering our knowledge of phage infection dynamics and bacteriophage-host interactions.
Comments
This presentation is currently embargoed. It will be available May 2029.