Presentation Type
Thesis
Department
Biology
Location
Walker Conference Center A
Description
Photodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the compound was then determined using HPLC. Additionally, ROS production using ZnTPP-IL was determined. The cytotoxicity of ZnTPP-IL was then examined by analyzing MTT assay with A549 lung cancer cell lines when paired with white and red light.
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Included in
Synthesis of ZnTPP-IL as a Potential Photodynamic Therapy Agent
Walker Conference Center A
Photodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the compound was then determined using HPLC. Additionally, ROS production using ZnTPP-IL was determined. The cytotoxicity of ZnTPP-IL was then examined by analyzing MTT assay with A549 lung cancer cell lines when paired with white and red light.