Incorporating a Bioengineered Protein and a Collagen Analog into Modern Wound Dressings
Presentation Type
Poster
Department
Biology
Location
Walker Conference Center B
Description
Collagen is a vital part of wound healing and has been incorporated into a variety of modern wound dressings including electrospun fiber mats. The limitations of human collagen include high costs and limited availability. Chitosan, another biopolymer used in wound healing, possesses antimicrobial properties, and offers protection agains biofilms which hinder healing of the wound bed. Previously, our lab has electrospun chitosan and PVA (poly(vinyl alcohol)) with collagen to produce fiber mats that have shown promise as modern wound dressings. Additionally, the Hamilton Lab has developed a cost-effective collagen analog that has been incorporated into nanofiber scaffolds. The resulting nanofiber mats have been designed to mimic the morphology of the extracellular matrix in the body for use in biomedical applications including wound healing.
These biomimetic electrospun scaffolds show promise for releasing molecules into the wound bed including proteins and other large molecules. Our lab has incorporated s-HFGF1 (super-human fibroblast growth factor), a bioengineered protein based on human fibroblast growth factor, into nanofiber mats. Studies have shown that the direct application of s-HFGF1 to cells results in increased proliferation in vitro. The protein paired with the biomimetic and antimicrobial properties of the novel nano fiber scaffolds should provide synergistic results including a faster wound healing as well as preventing bacterial infection. We have assessed the ability of our nanofiber mats to release the s-HFGF1 protein in physiological conditions. In the future, protein release studies will be performed in vitro and analyzed using cell migration assays. As a step towards cell studies, supernatants from these release studies will be used in NIH 3T3 viability assays to evaluate the ability of cells to survive exposure to these novel dressings. In the future, cell migration assays will be performed to determine the impact of the released protein and novel dressing on the rate of in vitro wound healing. It is anticipated that these experiments will further verify the release of biomolecules from novel nanofiber scaffolds and a synergistic healing effect will be observed.
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Incorporating a Bioengineered Protein and a Collagen Analog into Modern Wound Dressings
Walker Conference Center B
Collagen is a vital part of wound healing and has been incorporated into a variety of modern wound dressings including electrospun fiber mats. The limitations of human collagen include high costs and limited availability. Chitosan, another biopolymer used in wound healing, possesses antimicrobial properties, and offers protection agains biofilms which hinder healing of the wound bed. Previously, our lab has electrospun chitosan and PVA (poly(vinyl alcohol)) with collagen to produce fiber mats that have shown promise as modern wound dressings. Additionally, the Hamilton Lab has developed a cost-effective collagen analog that has been incorporated into nanofiber scaffolds. The resulting nanofiber mats have been designed to mimic the morphology of the extracellular matrix in the body for use in biomedical applications including wound healing.
These biomimetic electrospun scaffolds show promise for releasing molecules into the wound bed including proteins and other large molecules. Our lab has incorporated s-HFGF1 (super-human fibroblast growth factor), a bioengineered protein based on human fibroblast growth factor, into nanofiber mats. Studies have shown that the direct application of s-HFGF1 to cells results in increased proliferation in vitro. The protein paired with the biomimetic and antimicrobial properties of the novel nano fiber scaffolds should provide synergistic results including a faster wound healing as well as preventing bacterial infection. We have assessed the ability of our nanofiber mats to release the s-HFGF1 protein in physiological conditions. In the future, protein release studies will be performed in vitro and analyzed using cell migration assays. As a step towards cell studies, supernatants from these release studies will be used in NIH 3T3 viability assays to evaluate the ability of cells to survive exposure to these novel dressings. In the future, cell migration assays will be performed to determine the impact of the released protein and novel dressing on the rate of in vitro wound healing. It is anticipated that these experiments will further verify the release of biomolecules from novel nanofiber scaffolds and a synergistic healing effect will be observed.
Comments
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