Developing a Protocol for Bacteriophage Guided Evolution

Author

Hannah G. Matthews, Ouachita Baptist UniversityFollow

Date of Award

5-3-2024

Document Type

Thesis

Department

Biology

First Reader

Dr. Ruth Plymale

Second Reader

Dr. Nathan Reyna

Third Reader

Mrs. Jill Batson

Abstract

Experimentation was done to generate a protocol for expanding the host ranges of bacteriophages based on the Appelmans Protocol. Temperate bacteriophages were introduced to multiple species of Gordonia bacteria in a 96-well plate. Lysis was determined using spectrophotometer absorbance values for each well and compared to the known host range of each phage. The spectrophotometer results were compared at 48 hours post infection and 72 hours post infection, and 48 hours was found to be the optimal time post infection to record data. A ratio of 100 phage to 1 bacteria was effective for determining bacterial lysis in the protocol.

Comments

This thesis is currently embargoed. It will be released May 2029.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Matthews, Hannah G., "Developing a Protocol for Bacteriophage Guided Evolution" (2024). Honors Theses. 947.
https://scholarlycommons.obu.edu/honors_theses/947