Date of Award
4-17-2024
Document Type
Thesis
Department
Biology
First Reader
Dr. Joseph Bradshaw
Second Reader
Dr. Angela Douglass
Third Reader
Dr. Myra Houser
Abstract
Photodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the compound was then determined using HPLC. Additionally, ROS production using ZnTPP-IL was determined. The cytotoxicity of ZnTPP-IL was then examined by analyzing MTT assay with A549 lung cancer cell lines when paired with white and red light.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
White, Addison, "Synthesis of ZnTPP-IL as a Potential Photodynamic Therapy Agent" (2024). Honors Theses. 932.
https://scholarlycommons.obu.edu/honors_theses/932