Date of Award
Spring 2020
Document Type
Thesis
Department
Chemistry
First Reader
Dr. Joseph Bradshaw
Second Reader
Dr. Tim Hayes
Third Reader
Dr. Terry Carter
Abstract
Photodynamic cancer therapy (PDT) is a type of treatment involving the use of light in conjunction with a photosensitive agent- a chemical or series of chemicals designed for activation when exposed to light. This research project investigated the synthesis and identification of the novel photosensitive agent, H2TPP-Pro-OH. To create the water-soluble porphyrin, (S)-(+)-prolinol was reacted with the tetra-carboxyl porphyrin, H2TPPC, to form the final H2TPP-Pro-OH product. This compound was then purified using syringe filtration and column chromatography, and subsequently characterized using infrared (IR), nuclear magnetic resonance (NMR), and Ultraviolet-visible (UV-vis) spectroscopies, as well as High Performance Liquid Chromatography (HPLC). Finally, the utility of the material as a PDT agent was determined by examining the cytotoxicity of the H2TPP-Pro-OH product using an MTT assay on MDA-MB-231 triple negative breast cancer (TNBC) cells comparing dark and light exposure.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Shirley, Catherine, "Developing a New Water-Soluble Porphyrin as a Potential Photodynamic Cancer Therapy Agent" (2020). Honors Theses. 757.
https://scholarlycommons.obu.edu/honors_theses/757