Date of Award

2018

Document Type

Thesis

Department

Chemistry

First Reader

Dr. Joseph Bradshaw

Second Reader

Dr. Amber Chelette

Third Reader

Dr. Randall Wight

Abstract

Drug abuse has expanded from more well-known substances, such as cocaine and marijuana, to relatively new novel psychoactive substances. A group of these substances called synthetic cannabinoids have been increasing in usage throughout the 2000's, and these compounds carry significant and varying risks depending on the dose and composition of the synthetic cannabinoid. Patients have been observed having symptoms associated with high doses of synthetic cannabinoids when they take lower doses of the synthetic cannabinoid in addition to their antidepressant medication. In order to test the effects of co-administration of selective serotonin reuptake inhibitors (SSRis) and synthetic cannabinoids, mice were observed in a marble burying assay under the influence of the different drugs. Mice were given either fluoxetine (10-mg/kg), citalopram (20-mg/kg), or JWH-018 (0.1-mg/kg or 0.3-mg/kg) or a combination of the drugs. Mice without being injected with any of the drugs buried 6.375 marbles on average in the twenty-minute test. Mice injected with fluoxetine or citalopram alone buried 4.25 marbles and 4.0 marbles respectively. The JWH-018 doses were chosen to be ineffective so that the marbles buried at 0.1-mg/kg was 6.125 and the 0.3-mg/kg dose resulted in 6.375 marbles buried. Fluoxetine in combination with JWH-018 resulted in 3.0 marbles being buried for the lower dose and 1.875 marbles were buried with the high dose. Citalopram in combination with JWH-018 resulted in 3.625 marbles buried for the lower dose and 2.875 marbles buried for the high dose. When the SSRis were taken with an ineffective dose of JWH-018 a greater than anticipated drug effect occurred, since it occurred in both combinations it points to a pharmacodynamic effect instead of a pharmacokinetic effect.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.