Department
Biology
Document Type
Article
Publication Date
8-7-2012
Abstract
To improve usability of methods for quantifying environmentally persistent entomophthoralean resting spores in soil, we modified and tested two methods using resting spores (azygospores) of the gypsy moth pathogen Entomophaga maimaiga. Both methods were effective for recovering resting spores at concentrations >100 resting spores/g dry soil. While a modification of a method originally described by Weseloh and Andreadis (2002) recovered more resting spores than a modified method based on Percoll density gradients, the ability to estimate true densities from counts was similar for both methods. Regression equations are provided for predicting true resting spore densities from counts, with R 2 values for both methods P0.90.
Publication Title
Journal of Invertebrate Pathology
Publisher Statement
© 2012 Elsevier Inc. All rights reserved.
DOI
10.1016/j.jip.2012.07.021
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Hajek, Ann E., Plymale, Ruth C., and Reilly, James R. "Comparing Two Methods for Quantifying Soil-borne Entomophaga maimaiga Resting Spores," Journal of Invertebrate Pathology, Elsevier, 111:2 (2012) Aug 7, 193-195. doi: 10.1016/j.jip.2012.07.021