The Qualification and Standardization of Viral Particle Detection
Presentation Type
Thesis
Department
Physics
Location
Walker Conference Center C
Description
MicroRNAs are biomarkers for various clinical uses such as diagnosis, prognosis, treatment plans, disease risks, and progression. To better understand the implication and application of microRNAs, bacteriophage RNA was amplified and quantified to obtain quantitative plasmid data rather than on a virion basis. While plasmid isolation may be an extensive process, phage plasmid quantification produces reliable results and, therefore, can be used to create a standard of comparison. By standardizing this process, future phage analysis will have more statistical relevance, less quantification variance due to lysate factors, and the quality of the experiment can be assessed against the universal standard. All of these qualities can later be taken and applied to modern medicine to more efficiently identify, treat, and personalize the medical attention needed for each patient. Experimental techniques include plasmid amplification through the growth and isolation of competent E. coli cells, plasmid purification, and quantitative PCR procedure and analysis.
Creative Commons License
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The Qualification and Standardization of Viral Particle Detection
Walker Conference Center C
MicroRNAs are biomarkers for various clinical uses such as diagnosis, prognosis, treatment plans, disease risks, and progression. To better understand the implication and application of microRNAs, bacteriophage RNA was amplified and quantified to obtain quantitative plasmid data rather than on a virion basis. While plasmid isolation may be an extensive process, phage plasmid quantification produces reliable results and, therefore, can be used to create a standard of comparison. By standardizing this process, future phage analysis will have more statistical relevance, less quantification variance due to lysate factors, and the quality of the experiment can be assessed against the universal standard. All of these qualities can later be taken and applied to modern medicine to more efficiently identify, treat, and personalize the medical attention needed for each patient. Experimental techniques include plasmid amplification through the growth and isolation of competent E. coli cells, plasmid purification, and quantitative PCR procedure and analysis.
Comments
This work is currently embargoed until 2029.