Event Title

Effect of Lung Carcinoma Exosomes on the Tumor Microenvironment and Characterization of Contents as Novel Therapy

Presentation Type

Thesis

Department

Biology

Location

Walker Conference Center A

Description

Cancer uses intercellular communication to induce tumor growth and metastasis. Fibroblasts can become specifically transformed and associated with cancer to aid in the process. Extracellular vesicles, specifically exosomes, contain proteins, RNA, and DNA that are taken up by local cells to affect cellular behavior. Exosomes were isolated from lung carcinoma using total exosome isolation reagent and quantified. These exosomes were used to treat lung fibroblasts and enrich lung carcinoma cells, and the effects of exosomes on cancerous properties, cell viability and cell migration, were studied. An increase of cell viability was found with exosome treatment for both cell lines while migration only increased for enriched lung carcinoma cells. Additionally, mass spectrometry-based proteomics of exosomes isolated from hepatocellular carcinoma was analyzed. Through a survey of differentially expressed proteins between high and low grade hepatocellular carcinomas against normal liver cells, several pathways of interest were identified. Some common motifs in underexpressed genes were the regulations of cell signaling, cell metabolism, and cell death. A study of miRNAs in exosomes along with potential theraputic exosome uses was performed.

Comments

This presentation is currently embargoed; it will be available May 1, 2023.

This document is currently not available here.

Share

COinS
 
Apr 27th, 4:30 PM Apr 27th, 4:45 PM

Effect of Lung Carcinoma Exosomes on the Tumor Microenvironment and Characterization of Contents as Novel Therapy

Walker Conference Center A

Cancer uses intercellular communication to induce tumor growth and metastasis. Fibroblasts can become specifically transformed and associated with cancer to aid in the process. Extracellular vesicles, specifically exosomes, contain proteins, RNA, and DNA that are taken up by local cells to affect cellular behavior. Exosomes were isolated from lung carcinoma using total exosome isolation reagent and quantified. These exosomes were used to treat lung fibroblasts and enrich lung carcinoma cells, and the effects of exosomes on cancerous properties, cell viability and cell migration, were studied. An increase of cell viability was found with exosome treatment for both cell lines while migration only increased for enriched lung carcinoma cells. Additionally, mass spectrometry-based proteomics of exosomes isolated from hepatocellular carcinoma was analyzed. Through a survey of differentially expressed proteins between high and low grade hepatocellular carcinomas against normal liver cells, several pathways of interest were identified. Some common motifs in underexpressed genes were the regulations of cell signaling, cell metabolism, and cell death. A study of miRNAs in exosomes along with potential theraputic exosome uses was performed.