The Quantification and Standardization of Viral Particle Detection
Date of Award
4-22-2025
Document Type
Thesis
Department
Biological Science
First Reader
Dr. Nathan Reyna
Second Reader
Dr. Ruth Plymale
Third Reader
Dr. Chris Brune
Abstract
MicroRNAs are biomarkers for various clinical uses such as diagnosis, prognosis, treatment plans, disease risks, and progression. To better understand the implication and application of microRNAs, bacteriophage RNA was amplified and quantified to obtain quantitative plasmid data rather than a virion basis. While plasmid isolation may be an extensive process, phage plasmid quantification produces reliable results and, therefore, can be used to create a standard of comparison. By standardizing this process, future phage analysis will have more statistical relevance, less quantification variance due to lysate factors, and the quality of the experiment can be assessed against the universal standard. Experimental techniques include plasmid amplification through the growth and isolation of competent E. coli cells, plasmid purification, and quantitative PCR procedure and analysis. The standardization results of the experiment were inconclusive due to various factors, including negative control amplification, technical error regarding annealing temperatures, and lack of stability testing.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Malone, Jenna, "The Quantification and Standardization of Viral Particle Detection" (2025). Honors Theses. 966.
https://scholarlycommons.obu.edu/honors_theses/966
Comments
This thesis is currently embargoed until May 2029.