Date of Award


Document Type




First Reader

Dr. W. W. Everett


The purpose of this work was to study the kinetics of the binding of cupric ion to beef plasma albumin, in particular the decomposition of the Cu++--BPA complex which absorbs light at 375 millimicrons.

The work of Klotz, Urquhart, Klotz, and Ayers has shown that the characteristic absorption band at 375 mu produced by the binding of cupric ion to beef plasma albumin is due to a copper- sulfhydryl bond. They have further demonstrated that the disappearance of this absorption band with time is accompanied by irreversible changes in the structure of the protein. The intensity of the initial absorption was shown to increase with increasing pH up to about pH 8, where it appeared to decrease again, possibly because of the increased rate of decomposition of the complex at high pH.

Included in

Chemistry Commons



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