Steven G. Cresawn, James Madison University
Nathan S. Reyna, Ouachita Baptist UniversityFollow
Ruth Plymale, Ouachita Baptist UniversityFollow
Welkin H. Pope, University of Pittsburgh
Deborah Jacobs-Sera, University of Pittsburgh
Charles A. Bowman, University of Pittsburgh
Daniel A. Russell, University of Pittsburgh
Rebekah M. Dedrick, University of Pittsburgh
Tamarah Adair, Baylor University
Kirk R. Anders, Gonzaga University
Sarah Ball, The Ohio State University
David Bollivar, Illinois Wesleyan University
Caroline Breitenberger, The Ohio State University
Sandra H. Burnett, Brigham Young University
Kristen Butela, Seton Hill University
Deanna Byrnes, Carthage College
Sarah Carzo, Cabrini University
Kathleen A. Cornely, Providence College
Trevor Cross, Cabrini University
Richard L. Daniels, College of Idaho
David Dunbar, Cabrini University
Ann M. Findley, University of Louisiana at Monroe
Chris R. Gissendanner, University of Louisiana at Monroe
Urszula P. Golebiewska, Queensborough Community College
Grant A. Hartzog, University of California, Santa Cruz
J. Robert Hatherill, Del Mar College
Lee E. Hughes, University of North Texas
Chernoh S. Jalloh, University of KwaZulu-Natal
Carla De Los Santos, University of California, Santa Cruz
Kevin Ekanem, University of California, Santa Cruz
Sphindile L. Khambule, University of KwaZulu-Natal
Rodney A. King, Western Kentucky University
Christina King-Smith, Saint Joseph's University, United States
Karen Klyczek, University of Wisconsin-River Falls
Greg P. Krukonis, Gettysburg College



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Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.

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Copyright © 2015 the Authors



S1_Fig.pdf (3808 kB)
S1 Figure 1: Comparison of Cluster O genome maps

journal.pone.0118725.t001.ppt (44 kB)
S1 Table 1: Cluster O Mycobacteriophages

journal.pone.0118725.t002.ppt (33 kB)
S2 Table 2: ANI values for cluster O phages

journal.pone.0118725.g001.ppt (198 kB)
S1 Figure 2: Dotplot comparison of Cluster O mycobacteriophages

journal.pone.0118725.g002.ppt (146 kB)
S2 Figure 3: Cluster O mycobacteriophage virion morphologies

journal.pone.0118725.g003.ppt (139 kB)
S3 Figure 4: Genome map of Mycobacteriophage Corndog

journal.pone.0118725.g004.ppt (133 kB)
S4 Figure 5: Genome map of Mycobacteriophage Catdawg

journal.pone.0118725.g005.ppt (132 kB)
S5 Figure 6: Genome map of Mycobacteriophage Dylan

journal.pone.0118725.g006.ppt (134 kB)
S6 Figure 7: Genome map of Mycobacteriophage Firecracker

journal.pone.0118725.g007.ppt (128 kB)
S7 Figure 8: Genome map of Mycobacteriophage YungJamal

journal.pone.0118725.g008.ppt (176 kB)
S8 Figure 9: Conserved repeats sequences in the Corndog genome

journal.pone.0118725.t003.ppt (149 kB)
S1 Table 3: Corndog peptides identified by mass-spectrometry

journal.pone.0118725.t004.ppt (147 kB)
S2 Table 4: Identification of Catdawg proteins by mass spectrometry

journal.pone.0118725.g009.ppt (127 kB)
S9 Figure 10: Unusual translation initiation of the Corndog tape measure protein gene

journal.pone.0118725.g010.ppt (153 kB)
S10 Figure 11: Dylan MPME element

journal.pone.0118725.g011.ppt (116 kB)
S11 Figure 12: Sequence features of cluster O genomes

journal.pone.0118725.g012.ppt (72 kB)
S12 Figure 13: Insights into genome evolution



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