Date of Award
2012
Document Type
Thesis
Department
Chemistry
First Reader
Dr. Joe Jeffers
Second Reader
Dr. Martin D. "Marty" Perry
Third Reader
Dr. Randall Wight
Abstract
Podocytes are vital, specialized kidney cells that are post-mitotic and cannot proliferate. Nevertheless, regeneration of podocytes after low-level ablation from a presently unidentified stem cell pool has been observed. The objective of this study was to establish a mouse model for the study of this regeneration process. Two transgenic mice were developed, the first of which possessed a tomato-reporter transgene for the assessment of podocyte turnover. Adriamycin was used to induce podocyte damage in this mouse. The second transgenic mouse possessed the tomato-reporter transgene as well as transgene allowing for the diphtheria toxin inducible (iDTR) ablation of podocytes. Dosage tests were conducted to evaluate the effectiveness of these models in ablating podocytes. Both the Adrimycin and tDTR mouse models successfully induced podocyte damage, with the iDTR system being ~1500x more effective. The models exhibited podocyte damage far exceeding the threshold at which regeneration becomes impossible. Lower-level ablation is needed, and further iDTR dosage tests will be conducted to determine the optimal drug dosages.
Recommended Citation
Gomez, John A., "Establishing a Mouse Model for the Study of Podocyte Regeneration after Injury" (2012). Honors Theses. 48.
https://scholarlycommons.obu.edu/honors_theses/48